chipseq_pipeline icon indicating copy to clipboard operation
chipseq_pipeline copied to clipboard

Update bgd to bw conversion in callpeak_macs2.bds

Open florianduclot opened this issue 9 years ago • 2 comments
trafficstars

The bedgraph to BigWig conversion step throws an error on my system for all my files: (...)pooled.tagAlign.fc.signal.srt.bedgraph is not case-sensitive sorted at line 1879. Please use "sort -k1,1 -k2,2n" with LC_COLLATE=C, or bedSort and try again. As mentioned in the error message and at https://gist.github.com/taoliu/2469050, using LC_COLLATE=C fixes the issue on my system when the command is ran separately (outside of chipseq.bds pipeline).

Would this addition cause conflict with the rest of the code?

Thanks,

florianduclot avatar Jun 08 '16 16:06 florianduclot

The funciton already has LC_COLLATE=C in line 67 of https://github.com/florianduclot/TF_chipseq_pipeline/blob/ca31df6fb4f484156eb7c098be3f938158141498/modules/callpeak_macs2.bds

But it was not exported so I modified it to export LC_COLLATE=C (in the commit 082c4a7d4e73f6ba7469e3d8a0f7d099dcc782cf).

leepc12 avatar Jun 09 '16 00:06 leepc12

Sorry I didn't notice this line before.

Thanks!

florianduclot avatar Jun 09 '16 14:06 florianduclot