Compute matrix -a -b influence the signal

[Antony-1996]

When I use deepTools computeMatrix 3.1.2 to calculate the different TF binding signal around the peaks,I meet some problem. When we extend the -a -b region from 1kb,2.5kb,5kb,10kb,25kb to 50kb with the bin size also increase, the enrich pattern of different peaks changed.(python 2.7.15). Code: computeMatrix reference-point -S FOXA1.bigwig -R SE_enhancer.bed TE_enhancer.bed Open_region.bed -o Path_out/FOXA1.mat.gz --referencePoint center -b 1000 -a 1000 --binSize 10 -p 15 (total 2kb region bin 10) (total 5kb region bin 10) why these will happen,and we icontinue to increase the region length ,the signal for TE and OR continue to drop.

[dpryan79]

Oh, that's incredibly strange. Can you post your bigWig files somewhere so we can debug this?

[Antony-1996]

I downloaded bigwigs from Encode database. the Enocde ID for FXOA1 is ENCFF795BHZ.