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bitscore vs identity
Hi,
I see that minimum bitScore of a search to retain hits is 60 by default. I am working with custom databases and I notice in the annotations.tsv file that hits with similar identity can produce widely different bitscores. e.g.:
WP_023543351.1 WP_023543351.1 - Streptomyces niveus - [NiFe] Group 1h TRUE 0.94 1156.0
WP_011516268.1 WP_011516268.1 - Cupriavidus metallidurans - [NiFe] Group 3d FALSE 0.936 67.0
Is this purely due to the RBH being FALSE vs TRUE?
Before DRAM I searched for hits manually using diamond/mmseqs, and I used a minimum identity cutoff of 0.50 and query cover > 80%, but I'm not sure how that relates to the minimum bitScore used in DRAM?