Error message saying fasta location returns no paths

[dsgregoire]

Hello,

Prior to this, I have been able to use DRAm v 1.0 and 1.2 successfully with no issues. After downloading some genomes in .fa format and trying to run them through DRAM 1.2 today, I received the following error message:

14 fastas found 2021-07-20 20:06:37.762165: Annotation started Traceback (most recent call last): File "/opt/anaconda3/bin/DRAM.py", line 152, in <module> args.func(**args_dict) File "/opt/anaconda3/lib/python3.7/site-packages/mag_annotator/annotate_bins.py", line 969, in annotate_bins_cmd checkm_quality, rename_bins, keep_tmp_dir, low_mem_mode, threads, verbose) File "/opt/anaconda3/lib/python3.7/site-packages/mag_annotator/annotate_bins.py", line 999, in annotate_bins db_handler = DatabaseHandler(db_locs['description_db']) File "/opt/anaconda3/lib/python3.7/site-packages/mag_annotator/database_handler.py", line 17, in __init__ raise ValueError('Database does not exist at path %s' % database_loc) ValueError: Database does not exist at path None

I haven't changed anything with respect to the DRAM installation run prior and I checked this with v 1.0 and 1.2 and still get the same error message. It seems to be registering the fasta files properly but stalls afterwards. I've also tried running it with just .fa files in the directory to make sure and still get the same thing.

Any help on this would be much appreciated.

[rmFlynn]

It looks like somehow dram is looking for a database that is not set in its config. The fastest way to inspect the config is with DRAM-setup.py print_config, take a look at the output and see if the text Description db: is fallowed by a path, and that path exists. If you need to change the location of your databases, you can use DRAM-setup.py set_database_locations. More info on that sort of thing here.

Good luck, let us know how it goes.

[rmFlynn]

Hope it went well.