How to trim polyT from the front

[ayash338]

In RNA-seq data, some reads have poly A at the end, some reads have poly T at the front. How can I remove the polyT from the front of reads?

[sfchen]

Can you show me some reads here?

[ayash338]

Thank you for your reply. The following sequences are from my fastq file. TTTTTTTACCAAGGACAAGAATTACTGGTTCACATAAAACACAAGAGATCTGAAAACACACTTCAGAGGAACATAGCAGGAAAATCCTTTTTAGCGACTACAGCTAGTAGGATGCTTCACTGGAAATTGATAAAACAAGTGTTGCATCTTA TTTTTTTCAGTGTCTTGTAGCATGTCTTCTTCAGATAATCTGAAAATTGTGACAGGGCTACCCAAATTGATGTTATGATGATTGTCTTCAGAGATATCTCTATTTTGACCTTCCGAATCTTCTTCTGAAAGCTCTAGGTTTCTTTCAAAAG TTTTTTTGTGTATATAGACTTGCAGTTGCAAACCGAATTACATGCGTGGTGTCTCATAGACGTACAAATTCCACAGGCTGAGGCTTTCCTTTCTGCATCCAACCAAGCCCTGCGGTAATACATCTGGTCGACACGACAAAACCATGTTCAA TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTATATATATCTTATATACTTTTTTAAAATTAAAAAAAAAAC TTTTTTTTTTTTTTTTTTTTTTGGTGCAATGTTGGTTCTCTTTCTTTTAATTTTTACAAAGCTGGCAGCAGCATTCTTTAGCTATATACTTCTGTATGTAAAGTTGTAAAAAGTTTGGAGAAAAAAACTAGCATTGGTGACACAAAACTAT TTTTTTTCCAGTCCAAAAAAAAAAATCCCATCACTCTTTCAATCGGAGTTGCAATGGTTCGCTCAGCACAGTAACTTGTAAGCAAGCAAACAGAACATCAATAGCCAAGACTTTAGCATACGGAACCAGTACGCACTCCTGATACACGCGA TTTTTTTTTTTTTTTAGCAAGAATCAGACTTTAATGCATGACAGAATCAGATCTCCAATAAATAACACACCGCAGCATTTTACGTCTACATGCACAAAGGCAAAACACAAGAGAACGGCTTAACATCAGATGTCAAATGTTTTCCTACTCC TTTTTTTTTTTTTTCATAAAATCTCTGTAGATCGATAATGCAAAGAAACTTCTTACCAAAAAAAATACTATTAGCTTATATTGTTCCAAAAATAACCTAAAGCCCTAGCTAGAGTCTAAAGTAACTCGATGCCTCCATCTACTAGTTGGAG

I don't know much about RAN-seq, but when the cDNA fragment contains poly A and the 1st cDNA is sequenced, the poly T is read at the head, right? So I wish there is a command option which trim the polyT or polyX at the head. Thanks in advance.

[PEHGP]

+1