Using a already processed and normalized dataset.

[daacarri]

Hello again!

Sorry for the frequent questions.

I have everything working for data coming from cell ranger but would also like to use some of the tools in ascend with publicly available data sets.

I have a dataset from GEO Accession: GSE70630_OG_processed_data_v2.txt , that has cell barcodes, gene names and normalized counts.

It has been processed/normalized (I believe spike-ins are present however). I would like to use the differential expression, dimension reduction, cell cycle tools on this data set. But am running into problems as getting to PCA requires the other steps be completed prior.

I can get the data into an EMset format but thats really about it.

Any help would be appreciated! And sorry if this is a dumb question.