reg-gen
reg-gen copied to clipboard
Error in rgt-hint differential for my own data
Hello.
I have an issue during rgt-hint differential and it says:
Traceback (most recent call last): File "/home/kthbecu/.local/bin/rgt-hint", line 8, in
sys.exit(main()) File "/home/kthbecu/.local/lib/python3.10/site-packages/rgt/HINT/Main.py", line 95, in main args.func(args) File "/home/kthbecu/.local/lib/python3.10/site-packages/rgt/HINT/DifferentialAnalysis.py", line 119, in diff_analysis_run mpbs.read(mpbs_file) File "/home/kthbecu/.local/lib/python3.10/site-packages/rgt/GenomicRegionSet.py", line 254, in read io.read_to_grs(self, filename) File "/home/kthbecu/.local/lib/python3.10/site-packages/rgt/GenomicRegionSet.py", line 52, in read_to_grs with open(filename) as f: FileNotFoundError: [Errno 2] No such file or directory: ''
This issue is specific to my own data which is ATAC-seq on human (hg38), mapped by bowtie2 and peak-called by MACS3. rgt-hint differential on the tutorial data (DendriticCells) succeeded with no any error. How can I solve this issue?
Thank you in advance.