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Update RNAseq tutorial

Open kescobo opened this issue 3 years ago • 4 comments

Some work in this direction already in #2, though that's out of date now too. Also related to #3 .

kescobo avatar Jun 08 '22 15:06 kescobo

I made a draft for a new tutorial for RNAseq data. It uses reads from a TEX-treated sample in V. Cholerae and computes putative transcription start sites in it. It shows how to

  • download reads from SRA
  • align reads using bwa-mem
  • read .bam files and .gff files
  • annotate alignments with features from .gff files
  • compute coverage from alignments
  • compute maxima in the difference along the coverage as putative TSS

Does that make sense? I put everything here

maltesie avatar Apr 16 '23 12:04 maltesie

Go ahead an make a PR and we can discuss there. When you push to your fork, there should be a button that says "open pull request," but if not, you can open the pull requests tab here or on your repo, click "new PR", and then have your branch target main here:

image

kescobo avatar Apr 17 '23 21:04 kescobo

(if you'd like, I can kick off the PR from your fork, but it's good practice :-) )

kescobo avatar Apr 18 '23 13:04 kescobo

I created a PR, I think. Thanks for all the help :)

maltesie avatar Apr 18 '23 17:04 maltesie